AFDye 430 Picolyl Azide
作者:AFDye 430 Picolyl Azide 发布时间:2022-09-05 17:14:11
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分子量
705.71 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Yellow solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperatureAbs/Em Maxima: 430/537 nm
Extinction Coefficient: 15,000
Spectrally Similar Dyes: Alexa Fluor® 430, CF® 430
AZDye™ 430 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.