AFDye 546 Azide
作者:AFDye 546 Azide 发布时间:2022-09-05 17:14:11
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分子量
972.36 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperatureAbs/Em Maxima: 554/570 nm
Extinction Coefficient: 110,000
Flow Cytometry Laser Line: 532 nm
Microscopy Laser Line: 543 nm or 546 nm
Spectrally Similar Dyes: Alexa Fluor® 546, Atto™ 546, CF® 546
AZDye™ 546 Azide is a bright, orange-fluorescent dye that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
AZDye™ 546 (Alexa Fluor® 546 equivalent) is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye™ 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.
AZDye™ 546 dye is structural and spectrally identical Alexa Fluor® 546.