Cy3 Azide
作者:Cy3 Azide 发布时间:2022-09-05 17:14:11
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分子量
806.96 (protonated)
分子式
N/A
CAS
1782950-79-1
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Red solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperatureAbs/Em Maxima: 553/569 nm
Extinction Coefficient: 150,000
Flow Cytometry Laser Line: 532, 555 or 568 nm
Microscopy Laser Line: 532 or 555 nm
Spectrally Similar Dyes: Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
Cy3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine5 based fluorescent dyes.
This is sulfonated dye is also known as sulfo-Cyanine3.