AFDye 488 Picolyl Azide
作者:AFDye 488 Picolyl Azide 发布时间:2022-09-05 17:14:11
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分子量
763.69 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
纯度
>95% (HPLC)
外观
Yellow solid
储存环境
-20°C. Desiccate
运输条件
Ambient temperatureAbs/Em Maxima: 494/517 nm
Extinction Coefficient: 73.000
Flow Cytometry Laser Line: 488 nm
Microscopy Laser Line: 488 nm
Spectrally Similar Dyes: Fluorescein, Alexa Fluor® 488, CF® 488A, DyLight® 488, Atto™ 488
ZDye™ 488 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A.