DBCO-PEG4-Desthiobiotin,Desthiobiotin-PEG4-DBCO|CAS:2032788-37-5
作者:DBCO-PEG4-Desthiobiotin,Desthiobiotin-PEG4-DBCO|CAS:2032788-37-5 发布时间:2022-09-02 18:02:12
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中文:脱硫生物素-四聚乙二醇-二苯并环辛炔英文:DBCO-PEG4-Desthiobiotin,Desthiobiotin-PEG4-DBCOCAS:2032788-37-5产品参数1
Catalog #:
Y-PE-3039
Name:
DBCO-PEG4-Desthiobiotin
CAS:
2032788-37-5
Formula:
C39H53N5O8
MW:
719.9
Purity:
95%
Availability:
In Stock
Ships within:
24 hours
Storage condition:
-20℃
Shipping:
Ambient temperatureDBCO-PEG4-Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kdハ= 1011ハvs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin. DBCO is commonly used for copper-free Click Chemistry reactions.